| PEACS: Expression Profiling by qPCR | PEACS: Expression Profiling by qPCR |
| Results | The solution lies in a third key observation: the gene-expression profiles (vectors) of heterogeneous populations of cells are weighted linear combinations of the expression profiles (vectors) of the component states within the population, with the weights in this linear combination corresponding to cell-state proportions. |
| Results | Empirical p-values for PEACS scores were determined by Monte Carlo sampling: for a given perturbation with n replicates, a null distribution was obtained by randomly sampling n expression profiles from the experimental data, calculating a PEACS score, and iterating this process 10,000 times to generate a PEACS score null distribution. |