Analysis and statistics | For repeated measures of means, statistical difference was determined using Tukey’s honestly significant criteria, while a Student t-test (one or two-sample) was used for comparison of one or two values. |
Manipulation of membrane conductance using dynamic clamp alters voltage trajectories and modulation of input-output responses by voltage fluctuations | Further, voltage fluctuations were not able to significantly reduce rheobase values in the presence of -5 nS conductance (Fig 11Di; one sample Student t-test, P = 0.58), while with 15 nS rheobase significantly increased (Fig 11Di; one sample Student t-test , P <0.001). |
Manipulation of membrane conductance using dynamic clamp alters voltage trajectories and modulation of input-output responses by voltage fluctuations | With -5 nS conductance voltage fluctuations were not able to significantly increase firing rate from zero (Fig 11Cii; one sample Student t-test, P = 0.79), while these changes were significant under control and with 15 nS (Fig 11Cii; one sample Student t-test , P <0.001). |
Reducing voltage-dependence of membrane resistance reduces fluctuation-based modulation of input-output curves in stellate cells | The leftward shift in rheobase increased from 64.4 i 7.7 pA to 134 i 25 pA (Fig 9Ei; paired Student t-test, P = 0.001, n = 10), while the slope factor increased from 18.0 i 1.3 pA to 29.6 i 4.7 pA (Fig 9Eii; paired Student t-test , P = 0.02, n = 10). |
Stellate cell input-output functions are modulated weakly by membrane voltage fluctuations | As shown, voltage fluctuations induced a leftward shift in rheobase relative to conditions without fluctuations (Fig 1F; 56 i5.6 pA, P <0.001, Student t-test , n = 20) and resulted in spike-probability curves with an average slope of 26 i 8.4 pA (mean i s.e.m). |
Flexibility peaks are conserved and identify genes with decreased mRNA stability | Results were searched for the 175 ORFs with peak in 3’ UTR compared with all other ORFs; they show that these ORFs are characterized by significant lowering of both poly(A) halflife (t-test: p < 2.5 X 10—2) and overall halflife ( t-test : p < 1 x 10—2), indicating their production of unstable mRNAs (see Fig. |
Statistical analysis | The statistical significance of properties and classifications has been assessed by means of Fish-er’s exact test and t-test . |
Statistical analysis | A t-test is a statistical hypothesis test in which the test statistic follows a Student’s tdistri-bution if the null hypothesis is supported. |
OOPPCOOPC. | To estimate the error and sample size, we calculated the propagation of error in COOPu and COOPC, and used them in the student t-test to calculate statistical significance. |
OOPPCOOPC. | We calculated the t-value and degrees of freedom to complete the t-test . |
OOPPCOOPC. | We assumed significance for a p-value less than 0.05 for the two sample t-test . |