| Cell culture | Cell culture |
| Introduction | The most prominent actin structures in cells cultured on flat surfaces are stress fibers (SFs), which have been further classified into different subclasses (Fig 1A) [5,6]. |
| Introduction | TFM is the most direct and convenient method because it requires only small changes to standard cell culture protocols. |
| Regularlzation | (A) Schematics of a cell cultured on a soft elastic substrate with embedded fluorescent marker beads. |
| Acknowledgments | We thank Mrs. Barbe] Redlich for her excellent technical support and assistance in cell culture experiments as well as the Life Cell Imaging Center in Rostock, Sergei A. Kuznetsov and Dieter G. Weiss for their support in executing the wet-lab experiments. |
| Wet lab | Briefly, cells were cultured in laminin coated cell culture flasks and maintained at 37°C with 5% in media containing DMEM/ F12 supplemented with B27 media supplement, glutamine, heparin sodium salt and gentamycin (Invitrogen, Karlsruhe, Germany). |
| Wet lab | Cells cultured on coverslips were incubated with 0.5mM fluorescent Cholera Toxin B-Subunit (CT-B, Alexa 594) for 10 minutes at 4°C. |
| Wet lab | Briefly cells cultured were washed twice with phosphate-buffered saline (PBS) and lysed in 29 sodium dodecyl sulfate (SDS) sample buffer followed by subcellular fractionation. |
| Cell culture | Cell culture |
| Introduction | On the other hand, the CSNAP approach identifies consensus “chemotypes” from diverse chemical structures, which likely inhibit common targets capable of inducing similar phenotypes in cell culture . |
| Supporting Information | Determination of compound potency in cell culture , related to Fig. |