| Analysis of cell phenotype and cytokine secretion by flow cytometry | Analysis of cell phenotype and cytokine secretion by flow cytometry |
| Analysis of cell phenotype and cytokine secretion by flow cytometry | Following blocking of Fc receptors with antibodies, cells were labeled with CD 14 (PerCP-Cy5.5), CD1 1b (FITC), TIE-2 (Alexa 647), VEGFR-l (PE), TGFBR-l (Pacific Blue), TNF-Rl (Pacific Orange), CXCR4-, CCR5-, oc5l31-biotinylated specific antibodies (followed by strepta-vidin-Marina Blue) and analysed by flow cytometry using a Facs LSRII (BD Biosciences) equipped with a 610/20 nm filter on the violet detector. |
| Combining computational and experimental approaches to delineate the pathways controlling TEM pro-angiogenic function | TEM release of angiogenic factors measured by flow cytometry . |
| Identification of critical ligands impacting the phenotype and pro-angiogenic activity of TEM differentiated in vitro—Antagonistic effect of TG F-B and synergistic effects of TN F-or on TEM pro-angiogenic phenotype and function | First, changes in TEM phenotype were evaluated by flow cytometry 36h post treatment. |
| Patient and tissue specimens | TEM phenotype, cytokine secretion and pro-angiogenic activity were assessed by flow cytometry and in vivo or in vitro vascularization assay, respectively. |
| Supporting Information | TEM) cell populations were measured by flow cytometry 36 hours posttreatment and displayed as mean logz ratios relative to untreated cells. |
| TEM from peripheral blood and tumor tissue of breast cancer patients show distinct pro-angiogenic phenotypes | We characterized by flow cytometry the phenotype of TEM from patient peripheral blood and freshly dissociated tumor specimens obtained at time of surgery (see Material and Methods). |
| TEM from peripheral blood and tumor tissue of breast cancer patients show distinct pro-angiogenic phenotypes | Based on our immunostaining and flow cytometry protocol we observed that TEM did not constitute a distinct subset of monocytes. |
| TEM from peripheral blood and tumor tissue of breast cancer patients show distinct pro-angiogenic phenotypes | Expression levels of receptors and integrin measured by flow cytometry at the surface of TEM. |
| Comparison with FTTC | One of the most common techniques to reconstruct traction force based on TFM data is Fourier Transform Traction Cytometry (FTTC) [9,10]. |
| Displacement analysis and FTTC force reconstruction | From the displacement data, Fourier transform traction cytometry (FTTC) [9] was then used to estimate traction stress [10]. |
| Regularlzation | (C) Reconstruction of the traction forces with regularized Fourier Transform Traction Cytometry depends on the choice of a regularization parameter. |