| Analysis and statistics | For multiple comparisons, statistical significance was determined using either a one-way or two-way ANOVA . |
| Manipulation of membrane conductance using dynamic clamp alters voltage trajectories and modulation of input-output responses by voltage fluctuations | Changes in membrane conductance had a significant impact on the trajectory leading up to spike threshold (Fig 10A; one-way ANOVA , P <0.001, |
| Manipulation of membrane conductance using dynamic clamp alters voltage trajectories and modulation of input-output responses by voltage fluctuations | Changing membrane conductance also had a significant impact on the duration of the AHP associated With continuous firing at ~4 Hz (Fig 10C; one-way ANOVA , P <0.001, n = 9—12). |
| Manipulation of membrane conductance using dynamic clamp alters voltage trajectories and modulation of input-output responses by voltage fluctuations | Analysis of f-I curves indicated that gain was significantly modulated by changes in membrane conductance, but not by the introduction of membrane voltage fluctuations (Fig 11A and 11B; 2-Way ANOVA , P <0.001 for conductance, P = 0.36 for voltage fluctuations). |
| Reducing voltage-dependence of membrane resistance reduces fluctuation-based modulation of input-output curves in stellate cells | Application of TTX significantly reduced the gradual increase in input resistance across different voltages (2-way ANOVA , P <0.001, |
| Stellate cells express significant non-linear membrane properties leading up to spike threshold | Depolarizing stellate cells led to a progressive increase in steady-state membrane input resistance (Fig 2A; one-way ANOVA , P <0.001, n = 12). |