Of the 51 compounds predicted to be targeting microtubules, 36 compounds (71%) had more than 20% fold change in in-vitro tubulin polymerization assay and 14 had no measurable effect.

The endpoint absorbance based on change in OD (dOD) was used to quantify the degree of microtubule polymerization and was converted to percentage fold change relative to DMSO (0%).

The percentage fold change is listed for each compound.

The endpoint absorbance (dOD) was used to quantify the degree of microtubule polymerization and was converted to percent fold change (F) relative to DMSO drug vehicle (0%), as previously described (Fig.

Of the 51 compounds predicted to be targeting microtubules, 36 had more than 20% fold change in microtubule polymerization and 14 had no measurable effect (85B Fig).

The relationship between the relative and absolute abundance of a component can be understood in terms of fold change over time.

When total absolute abundance of mRNA stays constant, fold changes in both absolute and relative abundance of each mRNA are equal.

When total absolute abundance varies, fold changes in absolute and relative abundances of each mRNA are no longer equal and can change in difierent directions.