Index of papers in PLOS Comp. Biol. that mention
  • lysis
Lorenza A. D’Alessandro, Regina Samaga, Tim Maiwald, Seong-Hwan Rho, Sandra Bonefas, Andreas Raue, Nao Iwamoto, Alexandra Kienast, Katharina Waldow, Rene Meyer, Marcel Schilling, Jens Timmer, Steffen Klamt, Ursula Klingmüller
Mass spectrometry
Total cellular lysate was denaturated in 10% SDS and subsequently subjected to IP of ERKI
Protein array: Akt and ERK quantification
Cell lysates generated as for quantitative immunoblotting were diluted 1:16 in Array Buffer Plus (Array Buffer composed by 1% BSA, 0,5% NP40, 0,02% SDS, 50mM Tris pH7,4, 150mM NaCl, 1mM EDTA, 5mM NaF, 1mM Na3VO4) containing complete protease-inhibi-tor cocktail (Roche), supplemented with SDS and denatured.
Protein array: Akt and ERK quantification
Slides were incubated with calibrators and cell lysates shaking at 4°C overnight.
Protein array: Ras quantification
Cell lysates were diluted 1:10 with Array Buffer PLUS.
Supporting Information
B) Total cellular lysate was used to detect phosphorylation of MEK1/2, ERK1/2 and Akt on serine 473 and total actin used as normalizer.
Supporting Information
C) Total cellular lysate was used to detect p9ORSK phosphorylation on serine 227 and 380, total p9ORSK and protein disulfide-isomerase (PDI) used as normalizer.
Supporting Information
Total cellular lysate was used to detect MEK1/2 phosphorylation, total ERK, total MEK1/2 and total actin used as normalizer.
lysis is mentioned in 12 sentences in this paper.
Topics mentioned in this paper:
Hannah R. Meredith, Allison J. Lopatkin, Deverick J. Anderson, Lingchong You
Introduction
In other words, a low density population of moderate ESBL producers would lyse entirely because its collective ESBL concentration would be insufficient to inactivate the B-lactam, while a high density population would only eXperience partial lysis before its collective ESBL concentration can inactivate the B-lactam and promote the recovery of the surviving bacteria.
Introduction
Shortly after the antibiotic is first applied, the population will be reduced due to lysis and appear susceptible because it will not have yet benefited from the activity of ESBLs.
Introduction
Ideally, a treatment could pinpoint the time window when the most lysis has occurred and the least benefit has been experienced.
Model development and characterization
In this model, introduction of the antibiotic inhibits bacterial growth and causes lysis .
Model development and characterization
With a dense enough population, the collective intracellular and extracellular Bla, due to lysis or leaky secretion [50], will be sufficient to degrade the antibiotic to a sublethal concentration before all cells are eliminated (Fig.
Model development and characterization
If examined shortly after antibiotic treatment, the population will have just experienced significant lysis and will appear susceptible because the effects of Bla have not yet been fully recognized.
Predictive power of the recovery time for injection-based protocols
Once the initial antibiotic concentration is high enough to cause cell lysis (do > 0.5), the recovery time increases eXponentially with the initial antibiotic concentration until the antibiotic concentration is too high (610 > 10) and the recovery time becomes infinite.
Predictive power of the recovery time for injection-based protocols
This dependence is an intrinsic property of antibiotic-mediated lysis .
Predictive power of the recovery time for injection-based protocols
Under increasing concentrations of antibiotic (do > 10), the rate of antibiotic degradation is essentially saturated (limited by the population size and the constant production rate of Bla) and the recovery time is primarily determined by the lysis rate.
lysis is mentioned in 37 sentences in this paper.
Topics mentioned in this paper:
Juan Palacios-Moreno, Lauren Foltz, Ailan Guo, Matthew P. Stokes, Emily D. Kuehn, Lynn George, Michael Comb, Mark L. Grimes
Approach
Embeddings were first performed on data from cell lysates from 21 neuroblastoma cell lines grown in culture without treatments.
Cell Treatments and Fractionation
Phosphoproteomic analysis was performed on two endosome (E1, E2) and lysosome ( Lys ) fractions as shown in 810 Fig mass-density plots.
FYN and LYN Changed Intracellular Location upon RTK Stimulation
Increased FYN and LYN in faster sedimenting organelles ( lys and E1 fractions) likely reflects migration to multivesicular bodies, late endosomes, and possibly lysosomes [32].
Supporting Information
These data were used to define fractions shown on the graph at right ( lys , lysosomes; E1 and E2, endosomes).
lysis is mentioned in 4 sentences in this paper.
Topics mentioned in this paper: