| Discussion | However, a positive synergistic effect is predicted only for some combinatorial treatments suggesting robustness. |
| Inhibitor combination: model predictions and experimental validation | In some cases, we observed synergistic effects as the combination of inhibitors had a stronger effect on the readout than the sum of impact of the respective individual inhibitions. |
| Inhibitor combination: model predictions and experimental validation | To address the synergistic effect of the inhibitor combination treatment, we calculated the effect of the inhibitor combination compared to the single inhibitor treatment (Fig 8B). |
| Inhibitor combination: model predictions and experimental validation | Interestingly, we observed that the Ras inhibitor has a synergistic effect on pAkt and pERK with several other inhibitors such as Raf1, ERK and Rac inhibitors. |
| ANG-2 and PIGF survival analysis on breast cancer patients | 7C-E), suggesting that the synergistic effect of the corresponding pathways is required to have a significant impact on the survival. |
| Discussion | Furthermore, this study demonstrates that the synergistic effect of these treatments relies on crosstalk between TNF-Rl, VEGFR-l, TGF-B and TIE-2 pathways resulting in altered angiogenic activity (Figs. |
| Discussion | Moreover, the relapse free survival analysis showed a clear separation between patients with low and high expression for pro-angiogenic genes (ANG-2 and PIGF), and suggested that the synergistic effect of the corresponding pathways is required to have a significant impact on the survival. |
| Identification of critical ligands impacting the phenotype and pro-angiogenic activity of TEM differentiated in vitro—Antagonistic effect of TG F-B and synergistic effects of TN F-or on TEM pro-angiogenic phenotype and function | Identification of critical ligands impacting the phenotype and pro-angiogenic activity of TEM differentiated in vitro—Antagonistic effect of TG F-B and synergistic effects of TN For on TEM pro-angiogenic phenotype and function |
| The plasticity of TEM predicted computationally was validated experimentally using TEM differentiated in vitro | These results validate our in silico prediction and reveal the synergistic effect of TIE-2, VEGFR-l and TNF-oc pathways in controlling TEM pro-angiogenic activity. |
| Introduction | However, the discovery of synergistic effects is not trivial. |
| Introduction | However, it has been shown that under certain feedback conditions, strong synergistic effects will be seen between upstream and downstream points that are located serially along a pathway [7]. |
| Introduction | The second step in identifying useful combinations was to experimentally evaluate synergistic effects [29,30]. |