In this paper, emergent laws for describing mechanochemical systems are investigated for processive myosin-actin motility systems. On the basis of prior experimental evidence that longer pro-cessive lifetimes are enabled by larger myosin ensembles, it is hypothesized that emergent scaling laws could coincide with myosin-actin contact probability or system energy consumption. Because processivity is difficult to predict analytically and measure experimentally, agent-based computational techniques are developed to simulate processive myosin ensembles and produce novel processive lifetime measurements. It is demonstrated that only systems energy relationships hold regardless of isoform configurations or ensemble size, and a unified expression for predicting processive lifetime is revealed. The finding of such laws provides insight for how patterns emerge in stochastic mechanochemical systems, while also informing understanding and engineering of complex biological systems.
In these systems, levels of organization often emerge, as evidenced by cases Where cells form tissues, tissues form organs, and organs interact to form complete organisms. We hypothesized that that laws exist that describe system functioning at one level, independently of the configuration at other levels. The hypothesis was tested using simulations of motor protein systems, and demonstrated that patterns in their behavior emerge at a systems level. Results demonstrated a laW concerning energy utilization predicts the lifetime of these systems before dissociation, regardless of the components present in the system. These findings reveal organizational laws that simplify complex systems analysis and can facilitate engineering design approaches for bio-based technologies.
This general principal is highly applicable to biophysical systems, where complex system functionality emerges from stochastic mechanochemical molecular interactions [2—4]. Collective emergent functionality is a definitive feature of motility systems, where filament gliding behavior emerges from the interactions of myosin molecular motors and actin filaments [5,6]. To promote motility, myosins exert force as they stochastically attach and detach to gliding actin filaments [6—8]. However, it is not fully understood how changes in myosin isoform structure affect the system’s higher level functioning (e.g. how fast/long the filament continues gliding). Such considerations are important because many isoforms exist in the myosin superfamily, with particular isoforms suited for varied cellular functions including muscular contraction, cy-toskeleton scaffolding, and active diffusion . Better descriptions for how individual myosin structure  and ensemble size  relate to system functionality could promote understanding of both natural and engineered molecular motor systems [12,13]. Derived system laws that describe the operations at the systems level as components are altered could significantly advance analyses of natural and synthetic myosin performance [14,15], and have particular applications relating to myosin-based diseases such as cardiomyopathy, where muscle tissue growth is affected by individual myosin configuration . Additionally, such rules could aid in developing heuristics for engineered technologies such as nano-actuators, molecular materials, and bio-sensors [17,18].
Consistency in processivity is measurable through considering a system’s processive lifetime 73, which refers to the duration from initial myosin-actin contact until system dissociation occurs during periods when no myosins are in contact with actin (Fig 1) . Processivity is an essential metric to consider in the design of myosin-based nanotechnologies that operate on similar principles as motility assays . Motility assays are common experiments for investigating how individual myosin configuration affects system behavior, and these experiments often measure the velocity of actin filaments propelled by a bed of myosins . Typically, there is a negligible load assumed to act on the actin filament, which is representative of physiological situations with low external loads or nanotechnologies that operate in similar controlled environments. Although many models and simulations exist for myosin systems [5,22—24], they mostly concentrate on physiological models rather than motility assays. The simulation of motility assays, however, enables the experimental investigation of phenomenon such as how myosin isoform configuration affects the maximum achievable filament velocity and probability of contact among myosins and actin, which is suggestive of the potential loaded system capabilities. We therefore concentrate on building models and simulations of motility assays as a basis for investigating emergent system laws informed by fundamental biophysical experiments.
Because processivity is a system behavior that emerges from many myosins’ stochastic actions  , none of which are processive individually, it is not possible to predict 73 with certainty, but rather requires the consideration of an average. These complications have made quantifications of 73 difficult and established analytical models have instead relied on estimations [19,27] to predict whether a system behaves in an emergent
Schematic of processive myosin system with dissociation. Schematic of a myosin ensemble propelling actin at unloaded velocity vu. Myosin states are stochastic, with myosins being detached, attached and power-stroking (light yellow point of contact), or attached and drag-stroking (dark red point of contact). Initially three myosins are attached (top); laterthe filament has translated and one myosin is attached (middle); at processivity termination, all myosins are detached (bottom). regime  that enables perpetual processivity. These estimations require consideration of the contact probability of a system PC (the probability of at least one myosin being attached at a given time), as
These uncertainties arise from standard error in average filament velocity measurements at the systems level, and in determining specific behaviors of individual myo-sins, such as their rate of attachment which is not directly measureable. Because of these uncertainties and their propagation across scales, it is difficult to determine with precision the influence of particular parameters such as PC on system performance, since it is influenced by multiple measurements with uncertainty. Therefore, simulation approaches informed by empirical measurements can enable insights for how difficult to measure parameters affect system behavior.
As an alternative, we propose that system energy consumption E increases with the number of myosins in a system/ ensemble N, which assumes each myosin continues utilizing ATP at the same rate as more myosins are added to the system, and generally holds true for unloaded systems. It can then be hypothesized that such energy relationships could inform a scaling law, since it would support previous empirical measurements that processive lifetime increases as myosins are added to the system. With energy scaling, it is assumed that myosins consume energy from ATP at a rate e, and E = eN, which is a valid assumption if N does not influence e. Generally this assumption is true when adding more myosins to the system does not affect the individual force output of myosins. The cycling rate of myosins is primarily governed by the velocity of the gliding filament, and eventually saturates at higher velocities  , meaning that the linear assumption is valid for systems operating at high velocities. Such scenarios are possible when the force on the system is low, such as muscle contraction against low loads or in vitro motility studies where filaments must only overcome the drag force from their environment; in both cases, adding or removing myosins from the system does not significantly alter the gliding filament velocity. It is then possible to assume the energy required for perpetual processivity by approximating that PC % 90% when an average number of attached motors is NW 2 N - r m 2; a minimum required N for perpetual processivity occurs when N = 2/ r, or E = 2 - e/r if 73 scales with system energy.
Where empirical data is not available, analytical approaches are utilized to validate the predictions of the simulation through predicting the system behavior of the same system configuration input in both models. The unified expression would greatly aid in predicting mechanisms of complex in vivo biological systems while enabling rapid prototyping of myosin-based technologies . The isoform parameters chosen in this paper are the myosin lever arm length, myosin detachment rate, and myosin attachment rate which are three of the most critical parameters for optimizing myosins for nanotechnolo-gies. These parameters roughly correspond to the three primary phases of a myosin’s cycle consisting of a power-stroke (positive force generation), drag-stroke/detachment (negative force generation), and velocity dependent rate of binding to actin (Fig 1) . All three of these parameters have values investigated empirically for a number of different isoforms and their influence on motility behavior, which aids in validation of developed models and simulations.
The approach contrasts to past myosin simulations focusing on muscle [5,24,32] , which are challenging to validate for single myosin functioning. We have previously examined myosin force-velocity curves for varied isoforms and developed a discretized virtual motility assay environment . Here, analytical expressions and simulations are first examined for varied isoform types and validated with experimental evidence of how altered myosin molecular structures affect ensemble behavior. A computational environment is then built for simulating and measuring processive lifetime 73 durations, and these novel measurements are used to determine whether 73 scales with contact probability PC or system energy E modulation. Once a scaling metric is found that is independent of isoform configuration, an analytical expression is fit to the simulation data that serves as the unified expression for determining 73 of any system regardless of its components.
When a myosin is detached, it stores energy from ATP as strain while the myosin head is displaced from its equilibrium state and attaches to actin sites with attachment rate kon. Binding sites are spaced every xd (36nm) distance, based on actin’s highly conserved structure. Once attached, the myosin has displacement 5+ in its power-stroke, that decreases before reaching a point of zero-displacement based on lever length l and step angle 6, with 6+ = l - 5171(6), with 6 assumed as 30° for all isoforms considered in this study. Once a myosin reaches zero-displacement, it begins displacing in a negative direction as other myosins continue pulling the filament. The myosin detaches With rate constant kofic during its drag-stroke of length 6_ = v/koff. 6+, kc”, and koficwere determined as isoform parameters because they map to molecular structures that vary independently in nature [33,34] and are prevalent in myosin engineering experiments [14,35—37].
Time-average behaviors are validated from analytical and simulation predictions with experimental evidence from past unloaded motility assays, for varied isoforms. In unloaded assays, myosins propel the filament at unloaded velocity v“ and forces produced by power-stroking myosins balance drag-strokers, such that the time-average myosin displacement (d) equals zero. (d) is found by considering myosins with complete stroke lengths 60,1 2 6+ + 6_, leading to (d) 2 (6+/ 2) - (6+/60n) — suggesting that V” is linearly dependent on 6+ and kofic. In cases when motility systems operate under load, the time-average force is calculated by (f) = K - r - (d), Where the myosin stiffness K is found through considering that a myosin can not store energy greater than What myosins may approximately utilize from ATP (e = 6252]) to find K = e/6+2. A myosin’s duty ratio r is found analytically by modifying past methods  and assuming a filament has traveled x distance at time t, with a myosin head having probability p0n(x,t) and pofl(x,t) of attaching and detaching to binding sites, respectively. With rate constants for attaching kon(x) and detaching k0fi(x), a myosin’s interaction with actin When travelling at steady state is: probabilityWhen considering k0" as a high rate of attachment that occurs for a myosin head within a spatial proximity xz to a binding site, and that myosins only bind While detached, the probabili-ty of binding Pan over time to as a site passes is: Because binding sites are spaced regularly by xd, the average distance A a filament travels each myosin cycle is
The simulation operates in discrete spatial and temporal steps, with a filament translating dX = lnm each step over a duration dT determined by v = dX/dT, which is a small enough step size to capture individual myosin behaviors While keeping required computational effort to a minimum. Each myosin agent has three possible states of either (0) detached, (1) being attached to the filament during a power-stroke, or (2) being attached to the filament during a drag-stroke. During each timestep of the simulation, each myosin agent follows programmed logic as presented Fig 2A that is representative of a myosin’s mechanochemical states and behaviors. Depending on a myosin’s current state, it will begin following rules in one of three ‘Start’ blocks and continue through if/then statements until an ‘End’ command is reached. For instance, a myosin that is not bound to actin (state 0) will first check if a binding site is near, where xnb is the distance from a myosin’s zero strain location to the nearest binding site, xz represents how close a myosin head must be to a binding site to have a chance of binding, and step size 6+ represents the distance of a myosin head from the point of zero strain. If the check fails, the myosin ceases its actions until the next time step. If the check succeeds, a random number is generated and compared to a myosin’s chance of attachment for that time step to determine whether it binds and enters the power-stroke state for the next simulation step or ceases its actions. The chance to attach is based on the actin's attachment rate parameter kon and the window of time a binding site is available such that P(kon) 2 k0" - dT. If a myosin agent attaches, it remains in its power-stroke (state 1) until it has a head displacement d of zero, as its head translates with the travelling filament and has initial displacement d 2 6+ that reduces by dX each step. In the drag-stroke (state 2), a myosin has a random chance of detaching according to its detachment rate kofic and P(k0fic) = koff' dT. Fig 2B demonstrates a rendering of myosins operating as an ensemble according to the rules in Fig 2A.
Monte Carlo samplings were used to determine a myosin’s displacement at time t, and (f) is found from aggregating m measurements such that (f) = f (1‘), where f(t,-) is a mo-tor’s instantaneous force at time 1‘,- found by f = k - d. The simulation terminates once the standard error se of the mean for (1‘) reaches 56 g 0.005, therefore m varies each simulation. In these simulations velocity is considered an independent variable while force is a dependent variable, therefore iterative processes in assuming velocities is required in cases where the simulations are utilized to determine the velocity of a system under a specified load. In the case of motility assays, the external load is considered to be zero and an initial assumption for velocity is found through using the analytical equations.
The analytical and simulation models were validated by comparing empirical data  for chicken skeletal muscle myosin (kon 2 9005—1, koffz 16005—1, and 6+ 2 511m) under load to iso-forms with one configuration variable altered, while the remaining two are identical to chicken skeletal myosin as indicated in Fig 3A. The force-velocity relationship was found analytically through solving (1‘) = K‘ - r- (d) as described further in the methods section, while the simulated force-velocity relationship was determined through simulating ensemble systems at varied velocities and aggregating to find the time-average force until error was negligible.
Additionally, each isoform configuration parameter has unique influences on systems functioning, such as higher attachment rates leading to a greater force per velocity with no influence on maximum unloaded velocity, while step size and detachment rate decreases result in lower maximum velocities and force per velocity. These differences are important, because it suggests a complex relationship in emergent ensemble behavior based on individual myosin configuration. Notably, attachment rate increases result in greater energy expenditure in a system (because myosins cycle more often), while the other two configuration variables do not. The change in maximum velocity that results from alterations in myosin step size and detachment rate are a result of differences in how long a myosin remains in its power or drag-stroke state; in motility systems the total force of the system must equal zero, which suggests that myosins with longer drag-strokes or shorter power-strokes will generate more negative force or less positive force, respectively, therefore resulting in lower system filament velocities. Although there is limited empirical evidence describing the loaded response of synthetic iso-forms with altered variables in comparison to chicken skeletal muscle myosin, there is experimental evidence describing the maximum (or unloaded) velocity V” for synthetic myosins 
Analytically 1/” is found using Eq 2, while the simulation requires an a priori assumed velocity and determination for whether the time-average force of the system equals zero. The unloaded velocity for simulated systems was found by iteratively adjusting the input 1/ in increments of 100 nm/s from zero until (d) g Onm, and is plotted in Fig 3B in addition to the empirical results. There is strong agreement among analytical and empirical trends, suggesting that the biophysics is captured for each of the unique influences of isoform configuration inputs. The juxtaposition of these two modeling approaches is important, as the simulation determines relationships by allowing system relationships to emerge in contrast to explicit formulations from the analytical model, but both approaches result in similar predictions.
The analytical and simulation models are both extendible to predicting stochastic ensemble behavior, such as determining the probability that at least one myosin in the system is attached to actin, which is necessary for ensuring the system operates with a consistent trajectory and does not dissociate . The contact probability PC that describes the percentage of time that at least one myosin is attached to actin is used to find an adjusted unloaded filament velocity v: 2 PC - vu. To determine the simulated PC, Monte Carlo methods were used to count the number of attached myosins during runtime. Fig 4A and 4B are histograms for ensembles of N = 25 myosins and N = 100 myosins, demonstrating that occurrences obey a Poisson distribution, and there is a much lower chance of no myosins being attached as N increases.
Experimentally, data was collected using chicken skeletal muscle myosin and increasing the concentration of myosins added to a flow cell while measuring actin filament velocity, until adding more myosins to the system resulted in no further increases in motility velocity, which is indicative of the point when at least one myosin remains in contact with actin about 100% of the time  (Supplementary Movie 2 in 81 Text). In these experiments, a viscous liquid was added to the motility cell environment in order to reduce the chance of system dissociation when no myosins were attached, and enables the measurement of filament velocities relative to the highest possible velocity for the system (Fig 3C). This point was reached in experiments when amounts greater than 100 ug/mL of myosins were added to the motility cell. When simulation and experimental results are normalized for the case of chicken skeletal muscle myosins (kon 2 9005—1, koficz 16005—1, and 6+ 2 511m) as N varies, it was found that strong agreement occurs when plotted with N = 60 myosins as the ensemble size that corresponds to the maximum achievable velocity (Fig 3C), and reflects ensembles with a contact probability PC of about 91%. Uncertainty in these comparisons exist because it is not possible to determine with certainty the number of myosins interacting with actin filaments empirically, to measure the average filament velocity of the system with certainty, and because there are likely fluctuations in instantaneous filament velocities that occur physically, but are not represented in the models.
When NW 2 2, it is generally true that PC % 90% for chicken skeletal muscle myosin . It is possible to compare the simulation and analytical methods when considering many isoforms and N, to determine whether all systems of a given Na“ will have similar PC according to PC = 1 — (1 — r)N. The analysis is conducted by first choosing a median isoform (kon 2 20005—1, koficz 25005—1, and 6+ 2 10mm) and increasing the amount of myosins within the system until the average number of attached myosins is three, which results in a curve of NW and PC for that particular isoform (Fig 4D). The process was repeated for isoforms that have one isoform value different in comparison to the median isoform, which enabled a controlled basis of comparison to determine how each myosin isoform configuration input affected ensemble energy and processivity behavior. For instance, the curve “k0” 2 10005—1” in the legend of Fig 4D indicates ensembles of different sizes for an isoform with values of k0" 2 10005—1, koflz 25005—1, and 6+ 2 10mm. Isoforms were extrapolated one at a time for each isoform input parameter and resulted in a total of seven data sets that all followed the same curve in Fig 4D, thus indicating the basic relationship between the number of attached isoforms and probability that at least one was attached for all considered system configurations. The values of isoforms chosen for extrapolation in Fig 4D represent a set of isoforms within 2—3 times greater or smaller parameter values when compared to chicken skeletal muscle myosin, which forms a significant basis of comparison, while also being representative of known myosins with larger lever arms  and faster kinetics .
Therefore, either PC or Nat, could interchangeably scale with 73. Because 73 measurements are expected to scale exponentially with N as supported experimentally  , we propose a unified expression in the form 73(Natt) = AeB'Natt;, where Natt increases with N and r, and A and B are scaling constants. The exponential scaling assumption also follows from Fig 4A and 4B, because of the low chance of no myosins being attached in large ensembles. The scaling equation only holds if A and B have similar values for all isoforms, a property tested with simulations. If accurate, the equation enables the prediction 73 from any system based on Natt, which is a property of the system and not of single myosins and is difficult to validate with analytical and experimental approaches.
To determine these coefficients, the computational environment was modified to recreate processive lifetime events, with each simulation measurement reflecting the time from initial myosin-actin contact until system dissociation (Supplementary Movie 3 in 81 Text). Dissociation occurs in the simulation when no myosins are attached for lms, which represents an average duration before an actin diffuses from the myosins’ reach, based on past experiments . It is possible to manipulate this duration through altering the fluids viscosity in the environment. When histograms for processive lifetime 73 were produced from simulations of two different ensemble sizes N (Supplementary Movie 4 in 81 Text), they followed an exponential decay that agrees with past experiments  (Supplementary Section 1 in 81 Text). A sample processive run recorded from the simulation environment is presented in Fig 5, and illustrates a duration of time from initial contact among myosin and actin until system dissociation occurs when no myosins are attached for a period of greater than 1 ms.
A significant body of data was collected, beginning with the simulation of a median isoform configuration (km, 2 20005—1, koficz 25005—1, and 6+ 2 10mm) with an ensemble size N of 10 while myosin and system behaviors were recorded from the simulation. The ensemble size of the system was increased until the average processive lifetime exceeded 1s (higher values of processive lifetime began approaching perpetually proces-sive systems that required extensive computational effort). The process was repeated for isoforms that varied by one input variable in comparison to the median isoform (e.g. an iso-form with extrapolated kon = 10005—1 represented an isoform of kon 2 10005—1, koficz 25005—1, and 6+ 2 10mm). The extrapolation of one isoform variable from the median isoform enabled a controlled basis of comparison to determine how each myosin isoform configuration input affects ensemble energy and processivity behavior. Isoforms were extrapolated one at a time to produce seven curves that represented how each ensemble's contact probability PC corresponded to its processive lifetime in Fig 6A. Results in Fig 6A demonstrate an exponential increase in 73 that scales with system energy consumption (and therefore contact probability PC), as expected. However, the constants A and B are not conserved because myosins with higher detachment rates kofic have much lower 73
Processive myosin simulation rendering. The rendering illustrates six periods of time during the agent-based simulation of a single processive run-length event. In the first (top) frame, no myosins are attached, then myosins begin attaching and propelling the filament until a period of time greaterthan 1ms when no myosins are attached, which leads to systems dissociation (bottom frame). The duration of time recorded forthe run length event is measured from the initial point of myosin contact with a filament until system dissociation.
These results suggest that processive lifetime scaling for systems of different isoforms does not occur universally with contact probability, but could possibly occur through considering relationships with system energy consumption When considering that higher koficleads to increasingly unstable systems with higher energy requirements E (73) to reach a given 73 . The energy requirement arises from considering the average req
2 Natt(73) /r. It follows that a required system energy
The process is initiated by considering how Em] (73) fluctuates as each myosin parameter is altered independently. Eq 6 can be
- kofl, suggesting that Em] scales with kofic. This agrees with simulations because Nreq remains constant for myosins of varied step sizes 6+ (as duty ratio 1’ remains unchanged) While Ereq remains static as attachment rate kon varies despite e varying (Supplementary Section 2 in 81 Text). Em] was determined at 73 = 500ms by simulating increasingly larger ensembles until reaching the first occurrence of 73 2 500ms from an average of 1,000 simulations for a varied set of isoforms that includes a baseline isoform with k0” 2 12505—1, koficz 15005—1, and 6+ = 7.5mm, and isoforms with one input parameter extrapolated from the baseline isoform values (Fig 6B). Fig 6B demonstrates that Ereq grows with koficbut remains constant as other parameters vary. Therefore, Eq 6 is not a fully predictive model and the unified scaling law requires adjustment to account for the energy differences in processivity When koficis altered, Which suggests a modification to find an adjusted system energy E”< = E - Natt. sys
All isoforms have nearly identical E* while having vastly different contact probabilities, thus suggesting that E* is a viable predictor of processive lifetime (Supplementary Section 3 in 81 Text). Therefore, the unified expression that fits the simulation data is possible to express as 73(E*) = AeB'E*. When simulation results from Fig 6A are reconsidered with E*, there is strong agreement among all isoform types adhering to one master curve (Fig 7). Coefficients were fit to the median isoform in Fig 7, resulting in A m 14.5 and B % 4.5(10_4) and even hold as multiple parameters of isoforms are varied simultaneously (as represented by “low” and “high” isoform configurations in Fig 7). The results demonstrate that ensembles have similar processive lifetimes as a function of adjusted energy consumption E*, regardless of which isoform is present. These results are significant, because they suggest that the properties of the myosin motility system scale independently of the myosins configured within the system, despite individual changes in
Master curve that predicts processive lifetime of ensembles composed of many different myosin isoforms. Processive lifetimes for isoforms when adjusted system energy consumption E* varies. lsoforms are identical to Fig 6A, except for additional low (6+ = 5nm, k0,, = 10003—1, and keg: 15003—1) and high (6+ = 15nm, k0,, = 30003—1, and koff = 35003—1) isoforms, which demonstrate the master curve holds as multiple myosin parameters are altered. The master curve analytically captures the overall response predicted through the unified expression 73 = AeB‘E*, withA m 14.5 and B m 4.5(10‘4). Here, processive lifetime is predictable regardless of individual myosin configuration and the master curve asymptotes are indicative of energy thresholds for perpetual processivity. myosins having unique effects on other performance metrics as considered in previous figures (Figs 3A, 3B and 6A).
Two hypotheses were tested that were informed by past experimental data, that 73 scales with the number of myosins in a system or the probability that at least one is attached. Simulations demonstrated that neither of these hypotheses held true. Instead, a master expression was derived that enables novel quantifications of 73 through consideration of how the number of attached myosins influences energy consumption. The expression was determined through simulation evidence that although 73 always increases with larger contact probabilities and system energy for a given isoform, systems energy modulation potentially offers a more accurate scaling law that holds for all systems regardless of isoform presence. The unified expression simplifies understanding and analysis of myosin systems, as emergent system behavior is quantifiably independent of system sub-components.
The model makes several simplifying assumptions that could be further investigated as potential indicators of universal system behaviors. For instance, myosins have many different mechanochemical states and under certain circumstances, such as operating at very low velocities or high forces, other biophysical mechanisms may alter aspects of the three state cycle significantly. Additionally, the model makes a simplifying assumption that the system operates at a constant velocity and force regardless of how many myosins are attached to the filament; it is expected that since myosins may not contribute equal force to the filament over their entire cycle, the filament velocity would fluctuate with low myosin counts. These considerations suggest that the results of the unified master equation are applicable for the particular set of isoforms and assumptions modeled in this study, and further considerations of laws may be required to determine their applicability when the system enters differing regimes of emergent behavior  or operates under largely different conditions.
These situations were not investigated in the study as there is no validation for varied myosin isoforms experimentally. In the analytical and agent-based models of myosin, additional loads in the system would only alter the processive lifetimes of systems through reducing the filament velocity, since myosin cycles are dictated in the model entirely by the speed of the gliding filament. The reduction in filament speed would occur because myosins are required to balance forces among themselves and the external load. However, it is possible that empirical studies would show that pro-cessive myosin systems behave quite differently under load, because single myosins are no longer able to propel the filament at all, therefore possibly promoting a more stochastic instantaneous velocity.
The derivation of such laws is particularly important because they enable a simplification in the analysis of systems with many different parameters, and could form heuristics for engineers to follow when designing nanotechnologies. Through finding universal relationships in how the configuration of a system affects its behaviors, it enables researchers to concentrate on other tradeoffs present in the system, such as choosing among isoforms on the basis how fast they propel filaments under conditions that control for desired processive lifetimes across systems. Such considerations in simplifying the modeling and variables are crucial, both in promoting understanding from a scientific perspective and enabling effective development of bionanotechnologies.
Sl Text. Contains descriptions of Supplementary Movies, Supplementary Section 1 (Histogram of simulated processivity events), Supplementary Section 2 (Influences of Varied Isoforms on Processivity), and Supplementary Section 3 (Determination of a Unified Scaling Equation).
Performed the experiments: PE. Analyzed the data: PE. Contributed reagents/materials/analysis tools: PE IM. Wrote the paper: PE IC CS PL. Designed, coded, and operated molecular simulation: PE. Conceived and contributed to wet-lab experiments: PE IM.
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